light cycler r 480 (Roche)

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Roche light cycler r 480
Light Cycler R 480, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/light+cycler+r+480+system/LightCycler+480+System/pmc12957137-61-15-19
Average 99 stars, based on 6 article reviews

light cycler r 480 - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Insulin in combination with cisplatin induces the apoptosis of ovarian cancer cells via p53 and JNK activation.
Article Snippet: Samples were run on the Light Cycler R 480 system (Roche Diagnostics GmbH, Mannheim, Germany) using SYBR Premix Ex Taq (Takara Biotechnology, Co., Ltd.) according to the manufacturer's protocol.

Article Title: LncRNA-42060 Regulates Tamoxifen Sensitivity and Tumor Development via Regulating the miR-204-5p/SOX4 Axis in Canine Mammary Gland Tumor Cells.
Article Snippet: Finally, Light Cycler R©480 System (Roche, Basel, Switzerland) was used for quantitative analysis.

Membrane:

Article Title: Establishment and characterization of cisplatin-resistant cell lines from canine mammary gland tumors.
Article Snippet: The development of cisplatin resistance is one of the major causes of mammary cancer treatment failure, and is associated with changes in Sox4 gene expression.. To investigate the characteristic changes that occur in canine mammary gland tumor (CMGT) cells following the development of acquired cisplatin resistance, along with the relationship between these changes and the Sox4 gene.. We constructed cisplatin-resistant cell line, CHMpCIS, from the cell line CHMp, which was isolated from the primary lesion of a malignant CMGT.

SYBR Green Assay:

Article Title: Voluntary Exercise Improves Cardiac Function and Prevents Cardiac Remodeling in a Mouse Model of Dilated Cardiomyopathy.
Article Snippet: From 500 ng of extracted RNA, first-strand cDNA was then synthesized using the high capacity cDNA reverse transcription kit (Thermo Fisher Scientific, Saint-Herblain, France) with random primers according to manufacturer’s instructions. .. Using the Light Cycler R© 480 system (Roche Diagnostics, Meylan, France), the reaction was carried out in duplicate for each sample in a 6-μl reaction volume containing 3 μl of SYBR Green Master Mix, 500 nM of the forward and reverse primers each and 3 μl of diluted (1:25) cDNA. ..

Article Title: Absence of Desmin Results in Impaired Adaptive Response to Mechanical Overloading of Skeletal Muscle.
Article Snippet: From 500 ng of extracted RNA, the first-strand cDNA was then synthesized using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics) with anchored-oligo(dT)18 primer and according to the manufacturer’s instructions. .. Using a Light Cycler R© 480 system (Roche Diagnostics), the reaction was carried out in duplicate for each sample in a 6 μl reaction volume containing 3 μl of SYBR Green Master Mix, 500 nM of the forward and reverse primers each and 3 μl of diluted (1:25) cDNA. ..

Expressing:

Article Title: Long-Term Potable Effects of Alkalescent Mineral Water on Intestinal Microbiota Shift and Physical Conditioning.
Article Snippet: .. .e expression levels of uncoupling protein-1 (UCP-1) were quantitated from the total RNA extracted from adipose tissue around the mice testicles using the Light-Cycler R 480 System (Roche, KK, Japan). .. Primer sequences and Universal Probe Library (UPL) Probe (Roche) number are listed in Supplemental Table 2.

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